Lecture 1. Microbiology. Concept of Microbiology. History of Microbiology. Abundance and significance of microorganisms. Classification. The three domains. Taxonomy and phylogeny. Molecular clocks. Evolution and diversity of microorganisms.
Lecture 2. Structure and function of the prokaryotic cell. Morphology. Cytoplasm and prokaryotic organelles. Cytoplasmic membrane in prokaryotes. Prokaryotic cell walls. External structures. Prokaryotic mobility. The bacterial endospore.
Lecture 3. Microbial metabolism. Energy and carbon sources. Metabolic categories. Respiration. Fermentation. Prokaryotic photosynthesis. Chemolitotrophy. Autotrophy. Nitrogen fixation.
Lecture 4. Microbial growth. Cellular division in prokaryotes. Batch and continuous cultures. Factors affecting microbial growth. Microbial growth control. Culture media. The aseptic technique. Extremophiles. Strategies for survival.
Lecture 5. Genetics of prokaryotes. The prokaryotic genome. Chromosomes and plasmids. Genomics and metagenomics. Lateral gene transfer mechanisms: transformation, conjugation and transduction. Transposable elements. Genetic engineering.
Lecture 6. The Bacteria domain. Diversity and phylogeny of Bacteria domain. Applied and environmental significance. Phylum Cyanobacteria. Phylum Proteobacteria. Phylum Spirochaetes. Phylum Bacteroidetes. Phylum Actinobacteria. Phylum Chlamydiae. Phylum Firmicutes. Phylum Tenericutes. Other phyla.
Lecture 7. The Archaea domain. Diversity and phylogeny of Archaea domain. Applied and environmental significance. Phylum Euryarchaeota. Superphylum ‘DPANN?. Superphylum ‘TACK’.
Lecture 8. Microorganisms of the Eukarya domain. Eukaryotic microbes. Applied and environmental significance.
Lecture 9. Viruses. The ‘viriosphere’. General properties of viruses. Strategies of virus replication and synthesis of components. Prokaryotic and eukaryotic viruses. Viral lifecycles. Techniques for studying viruses. Applied and medical significance. Subviral agents.
Practical lesson (PL) 1. Preparation of cuture media
PL 2. Techniques of isolation, plating and incubation of microorganisms
PL 3. Ubiquity of microorganisms
PL 4. Sterility and contamination
PL 5. Microbial growth
PL 6. Effect of ventilation and antibiotics on microbial growth
PL 7. Bacterial identification
PL 8. Antimicrobial agents’ valuation
PL 9. Bacteriophage infection