Introduction. Molecular stage of life. Structure and function of proteins. Enzymes. Catalysis and enzyme kinetics. Carbohydrates. Lipids, biomembranes and membrane transport. Structure and function of nucleic acids. DNA Replication, repair and recombination. DNA Transcription and RNA maturation. Translation. Regulation of gene expression
THEORETICAL AND PRACTICAL CONTENTS:
B 1. INTRODUCTION.
T 1. Introduction. The Basis of Biochemistry.
1.1. Introduction to Biochemistry. 1.2. Water as a sustaining life medium. 1.3. Bioenergetics.
B 2. BIOMOLECULES.
T 2. Structure and function of proteins.
2.1. Amino acids. 2.2. Peptides. Primary structure determination. 2.3. Three-Dimensional structure and function of proteins.
T 3. Enzymes. Catalysis and enzyme kinetics.
3.1. Characteristics of biological catalysts. 3.2. Enzyme catalysis. 3.3. Enzyme kinetics. 3.4. Enzyme regulation.
4.1. Monosaccharides. 4.2. Oligosaccharides. 4.3. Polysaccharides. 4.4. Glycoconjugates.
T 5. Lipids.
5.1. Chemical nature of lipids. 5.2. Types and functions of lipids
T 6. Biomembranes and transport.
6.1. Structure of cell membranes. 6.2. Solute transport across cell membranes.
B 3. MOLECULAR BIOLOGY.
T 7. Structure and function of nucleic acids.
7.1. Nucleotides. 7.2. Structure and function of DNA. 7.3. Structure and function of RNA. 7.4. Genetic information.
T 8. DNA replication, recombination and repair.
8.1. DNA replication mechanisms. 8.2. DNA damage and repair.
T 9. DNA transcription and RNA maturation.
9.1. RNA synthesis. 9.2. RNA maturation mechanisms. 9.3. Regulation of transcription.
T 10. Translation.
10.1. Genetic code. 10.2. Protein synthesis.
PRACTICAL WORK: P 1. Reagents preparation. P 2. Catalase enzymatic activity. P 3. Quantitative estimation of proteins. P 4. Enzyme activity measurement of polyphenol oxidase. P 5. DNA isolation from halophilic Archaea. Agarose gel electrophoresis. P 6. Gel-filtration chromatography. P 7. Isolation of casein and lactose from milk.